Improving the Oxidative Stability of a High Redox Potential Fungal Peroxidase by Rational Design
Fig 4
Inactivation kinetics under oxidative conditions.
A, Native VP and single and double methionine variants: native VP (●), M247F (○), M152V (■), M265L (□), M262F (▲), M262F/M265L (♦); and B, native VP and multiple variants: native VP (●), T45A/I103T (○), M152F/M262F/M265L (■), M152F/M247F/M262F/M265L (□) and T45A/I103T/M152F/M262F/M265L (▲). Reactions were carried out at 25°C using 0.01 μM enzyme in 10 mM sodium tartrate (pH 5.0) in the presence of increasing H2O2 concentrations. Means and 95% confidence limits are shown.