Single Chain Variable Fragment Against Aβ Expressed in Baculovirus Inhibits Abeta Fibril Elongation and Promotes its Disaggregation
Fig 4
Disaggregation of mature Aβ fibrils (48 h incubation) by anti-Aβ scFv.
(A) TEM images of the disaggregation of Aβ fibrils in the absence (a, c and d) or presence (b) of anti-Aβ scFv. (a) TEM image of A42 fibrils incubated in boric acid buffer for 48 h; (b) A42 fibrils from (a) were treated with the scFv [VL-(G4S)3-VH-His] for 48 h; (c) A42 fibrils from (a) were treated with scFv buffer for 48 h; (d) A42 from (a) was continuously incubated in boric acid buffer for a total of 96 h; (scale bar = 250 nm); (B) The histogram shows the corresponding lengths of the fibrils from each group mentioned in (A). **: p<0.01.