Advanced Glycation End-Products Induce Apoptosis in Pancreatic Islet Endothelial Cells via NF-κB-Activated Cyclooxygenase-2/Prostaglandin E2 Up-Regulation
Fig 5
Advanced glycation end-products (AGEs) induced the production of prostaglandin-E2 (PGE2).
MS1 cells were preincubated with NS398 (a cyclooxygenase-2 inhibitor, 10 and 20 μM) for 30 min, and then treated with AGEs (200 μg/mL) for 24 h. Bovine serum albumin (BSA, B, 200 μg/mL) was as a negative control. The cell culture medium subjected to PGE2 testing, and the PGE2 concentration was normalized to the amount of cellular protein to which the conditioned media was exposed. Data are presented as means ± SEM from three independent experiments. *P < 0.05 vs BSA; #p < 0.05 vs. AGEs.