Metabolic Dysfunction and Altered Mitochondrial Dynamics in the Utrophin-Dystrophin Deficient Mouse Model of Duchenne Muscular Dystrophy
Fig 5
Increased expression of glycolytic enzymes in DKO muscles.
A) Western blots depicting higher levels of glycolytic enzymes DKO EDL compared to WT. B) HK1 protein level normalized to GAPDH is significantly higher in DKO EDL compared to WT (p = 0.0063) C) PK M2 protein level normalized to GAPDH is significantly higher in DKO EDL compared to WT (p = 0.0001). D) Western blot showing key metabolic regulators are unchanged in DKO EDL compared to WT. E) Western blots showing glycolytic enzymes protein levels are higher in DKO diaphragm. F) HK1 protein level normalized to GAPDH is significantly higher in DKO diaphragm compared to WT (p = 0.0004) and G) PK M2 protein level normalized to GAPDH is significantly higher in DKO diaphragm compared to WT (p = 0.0066). H). Glycolytic enzymes protein expression levels in ventricle. p< 0.05 is significant. * = p<0.05, ** = p<0.01 and *** = p<0.001. HK 1- Hexokinase 1, HK 2- Hexokinase 2, PK M1- Pyruvate kinase M1, PK M2- Pyruvate kinase M2, CS- Citrate synthetase, TFAM- Mitochondrial transcript factor A, LDH- Lactate dehydrogenase.