Engagement of Fas on Macrophages Modulates Poly I:C Induced Cytokine Production with Specific Enhancement of IP-10
Fig 2
Activation of Fas decreases poly I:C-induced IL-8, IL-10, TNFα and IFNβ expression.
THP-1-derived macrophages were treated with 100 ng/ml CH11 for 1 hr followed by stimulation with 20 μg/ml poly I:C. Changes in cytokine expression were detected by qRT-PCR after 8 hrs (A, B) and by ELISA after 48 hrs (C). Human monocyte-derived macrophages from three separate donors were treated with 100 ng/ml CH11 for 1 hr followed by stimulation with 20 μg/ml poly I:C. Changes in cytokine expression were detected by qRT-PCR after 8 hrs (D) and by ELISA for IP-10 after 48 hrs (E). Data shown are a combination of three independent experiments, with values shown as Mean ± SEM. * p<0.05, ** p<0.01 and *** p<0.001.