Anti-Yo Antibody Uptake and Interaction with Its Intracellular Target Antigen Causes Purkinje Cell Death in Rat Cerebellar Slice Cultures: A Possible Mechanism for Paraneoplastic Cerebellar Degeneration in Humans with Gynecological or Breast Cancers
Fig 7
Rat cerebellar slice culture incubated with serum from Patient 3, a neurologically normal individual with mixed mesodermal ovarian sarcoma.
Panels A and B demonstrate. extensive accumulation of IgG not only within Purkinje cells (arrow) but also in other neurons within and near the Purkinje cell layer (asterisks). Panels C and D: Purkinje and other neurons exhibiting Cy5 labeling for human IgG do not contain SYTOX green, indicating that IgG uptake had occurred in viable cells. Scale bars = 20μ.