Evolutionarily Conserved 5’-3’ Exoribonuclease Xrn1 Accumulates at Plasma Membrane-Associated Eisosomes in Post-Diauxic Yeast
Fig 1
Expression of Xrn1-GFP fusion rescues the xrn1Δ phenotype.
Standard spot tests comparing the growth rates of the wild type strain (wt in A; strain CRY155), xrn1Δ cells (Δ in A; strain CRY1233) and a strain expressing Xrn1-GFP (GFP in A; strain CRY1703) on SC (left panel) and YPD media (right) are shown. Cellular distributions of Dcp2-RFP in the three backgrounds are compared (wild type—B, xrn1Δ—C, and a strain expressing Xrn1-GFP—D; strains CRY2238, CRY2241 and CRY1396, respectively). Wide-field fluorescence of Dcp2-RFP and DIC channels are presented in B-D. Bar: 5μm.