Involvement of Histidine Residue His382 in pH Regulation of MCT4 Activity
Fig 1
Inhibitory effects of metals on MCT4-mediated lactate uptake.
(A) MCT4-mediated uptake of 0.1 mM lactate (0.1 μCi/ml) was measured in a transport buffer of pH 7.5 containing 5 mM of each metal for 10 min. The inset shows the effect of 5 mM zinc under an acidic condition (pH 5.5). (B) MCT1-mediated uptake of 0.1 mM lactate (0.1 μCi/ml) was measured in a transport buffer of pH 7.5 containing 5 mM of each metal for 10 min. The background uptake values of water-injected oocytes were subtracted. Data are presented as means±S.E. of three experiments.