Characterization of Effector and Memory T Cell Subsets in the Immune Response to Bovine Tuberculosis in Cattle
Fig 5
Proliferation of cell subsets in response to mycobacterial antigens.
Approximately 7 weeks after M. bovis challenge, PBMCs from infected cattle were either long or short-term cultured (6 days). Long-term cells consisted of PBMCs cultured in the presence of rAg85A, rTB10.4, rESAT-6:CFP10 and PPDb for 13 days (n = 4). Subsequently, cells were stained with CellTrace violet dye and re-stimulated with either rESAT-6:CFP10 or medium in the presence of APCs for an additional six days. Short-term cells consist of CellTrace Violet-stained PBMC from M. bovis-infected cattle cultured for six days in the presence of either rESAT-6:CFP10 or medium (n = 4). Proliferative responses of cells under both culture conditions were compared for each T cell subset. Data are presented as mean fluorescence intensity (MFI, y-axis) of CellTrace Violet for analysis of proliferation of T cell subsets (x-axis). Two-way ANOVA, n = 6, Šídák’s multiple comparison post-test.