Combining Microfluidics, Optogenetics and Calcium Imaging to Study Neuronal Communication In Vitro
Fig 1
Design of a simple neuronal device.
A- Scheme showing the neuronal device featuring two micro-wells seeded with hippocampal neurons (DIV 15) and connected by an array of axon diodes. The neurons colored in green correspond to neurons that are transduced by ChR2. Neurons colored in purple are not transduced. B- Image of a neuronal device obtained by confocal fluorescence microscopy showing neurons expressing ChR2-YFP (false color).