Deficiency of Angulin-2/ILDR1, a Tricellular Tight Junction-Associated Membrane Protein, Causes Deafness with Cochlear Hair Cell Degeneration in Mice
Fig 8
Compensatory accumulation of angulin-1/LSR in the organ of Corti in Ildr1k-/- mice.
A. Immunofluorescence microscopic images of the organ of Corti in the middle turn in wild-type (+/+) and Ildr1k-/- (-/-) mice at various time points using anti-angulin-1/LSR mAb (green) and anti-occludin pAb (red). Note that the angulin-1/LSR signals are evident at TCs in the organ of Corti in Ildr1k-/- mice, but not in wild-type mice. B. The fluorescence intensities of angulin-1/LSR and occludin at TCs in the organ of Corti in wild-type mice and Ildr1k/- mice at P10 were quantified. Error bars indicate s.d. *p<0.005 (Student’s t-test). Note that the intensity of angulin-1/LSR was significantly up-regulated at TCs in Ildr1k/- mice, whereas that of occludin was unchanged. At least two wild-type mice and Ildr1k-/- mice were analyzed for each time point and consistent results were obtained. Bars, 10 μm.