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Deficiency of Angulin-2/ILDR1, a Tricellular Tight Junction-Associated Membrane Protein, Causes Deafness with Cochlear Hair Cell Degeneration in Mice

Fig 4

Apoptotic degenerative death of hair cells in Ildr1^k-/- mice.

Immunofluorescence microscopic images of the organ of Corti in the middle turn in wild-type (+/+) and Ildr1^k-/- (-/-) mice at P10 and P12 using anti-cleaved caspase-3 antibody (green) and phalloidin (red). At P10, cleaved caspase-3 signal, which indicates apoptotic cells, was detected in the organ of Corti neither in wild-type nor in Ildr1^k-/- mice. At P12, cleaved caspase-3 signal was hardly detected in wild-type tissue whereas prominent signals were detected in the OHCs and IHCs in Ildr1^k-/- mice. Bar, 20 μm.

doi: https://doi.org/10.1371/journal.pone.0120674.g004