Development and Characterization of Recombinant Antibody Fragments That Recognize and Neutralize In Vitro Stx2 Toxin from Shiga Toxin-Producing Escherichia coli
Fig 1
A. RNA extraction from Stx2 IgG-producing hybridoma. Molecular marker (lane 1); Total RNA from anti-Stx2 IgG-producing hybridoma (mAb 2E11) (lane 2). B. scFv gene cloning confirmation. Molecular marker (lane 1); PCR product (lane 2). C. scFv fragment purification. Molecular weight ladder (lane 1); scFv elutions (lanes 2 and 3). D. Phage gene cloning confirmation. Molecular marker (lane 1); Double digestion (lane 2). Phage ELISA, results of independent experiments, performed in triplicate, are expressed as the means ± SEM * p < 0.05 compared with control. E. Electrophoretic analysis of Fab fragment purification. Molecular weight ladder (lane 1); Fab fragment purified (lane 2).