Regulation of Autoimmune Germinal Center Reactions in Lupus-Prone BXD2 Mice by Follicular Helper T Cells
Fig 3
Tfh cell responses were increased in the BXD2 mice.
(A) Flow cytometry analysis of the percentage and number of PD-1+CXCR5+ CD4+ T cells in the spleens of WT and BXD2 mice at the age of 6 months or older. (B) Quantitative RT-PCR analysis of Tfh cell related genes from WT and BXD2 mouse splenocytes at the age of 6 months or older. (C) Flow cytometry analysis of the percentage and number of CD4+Foxp3+PD-1+CXCR5+ Tfr cells in the spleens of WT and BXD2 mice at the age of 6 months or older. (D) Ratio of Foxp3- Tfh cells to FoxP3+ Tfr cells in the mouse spleens and germinal center B cells to FoxP3+ Tfr cells in the mouse spleen. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.