Isorhamnetin Attenuates Atherosclerosis by Inhibiting Macrophage Apoptosis via PI3K/AKT Activation and HO-1 Induction
Fig 2
Protective effects of Iso against ox-LDL-induced THP-1-derived macrophage cell injury.
THP-1 monocytes were incubated with PMA (160 nM) to induce differentiation into macrophages. (A) Effects of ox-LDL on macrophage cell viability. Cells were treated with increasing concentrations of ox-LDL (20, 40, 60, 80, 100, 120, and 140 μg/ml) for 24 h, and cell viability was determined by MTT assay. (B) Effects of Iso on ox-LDL-induced macrophage cell viability. Macrophages were pretreated with indicated doses of Iso and LOV for 8 h before exposure to 100 μg/ml ox-LDL for 24 h. Cell viability was measured by MTT assay. (C) Effects of Iso on macrophage cell viability. Cells were incubated with the indicated doses of Iso and LOV for 8 h. Cell viability was measured by MTT assay (A, B and C, expressed as the percentage of control). All results are expressed as the mean ± SD of three independent experiments. ##P < 0.01 vs. control group; *P < 0.05, **P < 0.01 vs. ox-LDL-treated cells.