A Novel Human Mutation in the SLC9A1 Gene Results in Abolition of Na+/H+ Exchanger Activity
Fig 1
Analysis of expression and targeting of wild type (WT) NHE1 and mutant (N266H) protein.
A, Western blot of whole cell lysates of stable cell lines expressing WT Na+/H+ exchanger or N266H mutant protein. 100 μg of total protein was loaded in each lane. The sample was immunoblotted with anti-HA tag antibody. The arrow indicates the position of full length glycosylated NHE1 protein. AP-1 is a cell lysate from mock transfected AP-1 cells. Results are typical of 5 stable cell lines. B, Surface localization of NHE1 in AP-1 cells expressing WT and N266H mutant. Equal amounts of total cell lysate (T) and unbound intracellular lysate (U) were examined by Western blotting with anti-HA antibody to identify NHE1 protein as described in the “Materials and Methods”. WT and N266H are cell lines stably expressing wild type NHE1 and mutant NHE1 respectively. Results are the mean ± the S.E. n = at least 3 determinations. The arrow indicates the position of full length glycosylated NHE1 protein.