SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor
Fig 3
SNAP23 but not other SNAP homologs regulate VWF exocytosis.
(A) The effect of siRNA targeting individual SNAP homologs. HUVEC were transfected with siRNA against SNAP homologs or control siRNA, stimulated with media or histamine, and VWF release was measured by an ELISA. Knockdown of SNAP23 decrease stimulated VWF release, but knockdown of other SNAP homologs has no effect on VWF release (n = 4–6 ± S.D.). (B) Comparison of the role of SNAP23 and SNAP29 in mediating exocytosis. The effect of SNAP23 and SNAP29 in endothelial exocytosis after RNAi was measured as above. Knockdown of SNAP23 decreased exocytosis, but knockdown of SNAP29 had no effect (n = 14–16 ± S.D.).