SNAP23 Regulates Endothelial Exocytosis of von Willebrand Factor
Fig 1
SNAP23 is expressed in human endothelial cells and murine tissues.
(A) Heat map of the gene expression values for SNAP homologs in 6 samples of human umbilical vein endothelial cells (HUVEC) by microarray. Relative expression values were normalized to that of GAPDH. (B) Relative expression of SNAP homologs in 3 individual donors of HUVEC as assayed by RT-qPCR. Expression was normalized as percentage of SNAP23 expression. Primers listed in Table 1. (C) ENCODE data on the UCSC genome browser depicting the expression of HUVEC SNAP homologs as assayed by RNA-seq. (D) SNAP23 is expressed in human brain microvascular endothelial cells (HBMEC), human aortic endothelial cells (HAEC), and HUVEC as measured by Western blot. (E) SNAP23 is expressed in multiple murine tissues as measured by qRT-PCR (n = 3 ± SD).