The Crystal Structure and Small-Angle X-Ray Analysis of CsdL/TcdA Reveal a New tRNA Binding Motif in the MoeB/E1 Superfamily
Fig 1
Cyclic N6-threonylcarbamoyladenosine (cyclic t6A, ct6A) hypermodification in tRNA.
a Schematic cloverleaf representation of tRNALysUUU showing the location of the ct6A modification at A37. b TcdA catalyzes the ATP-dependent cyclization of t6A into ct6A in tRNAs with ANN anticodons in bacteria, yeasts, fungi and plants, which occurs mainly at the A37 position of the anticodon stem loop (ASL) of the tRNA molecule. (c) Recognition of the AAA codon on mRNA by ASL of tRNALysUUU involves interactions between t6A37 and A1 from the AAA codon (PDB 1XMO).