Investigating the Causes for Decreased Levels of Glutathione in Individuals with Type II Diabetes
Fig 1
Assay of total and rGSH in RBCs, plasma, and monocytes from healthy subjects and individuals with T2DM.
RBCs, plasma, and monocytes were isolated by density centrifugation from peripheral blood drawn from healthy volunteers and individuals with T2DM. GSH assay was performed using a colorimetric assay kit from Arbor Assays. Total GSH in RBCs isolated from individuals with T2DM was significantly lower compared to the healthy individuals (Fig. 1A). There was an observable decrease in rGSH in RBCs isolated from individuals with T2DM compared to healthy volunteers (Fig. 1B). Total GSH in plasma samples from individuals with T2DM was significantly lower compared to the healthy individuals (Fig. 1C). There was a significant decrease in rGSH in plasma samples from individuals with T2DM compared to healthy volunteers (Fig. 1D). Total GSH in monocyte samples isolated from individuals with T2DM was significantly lower compared to total GSH measured in monocytes from healthy individuals (Fig. 1E). There was a significant decrease in rGSH in monocyte samples isolated from individuals with T2DM compared to healthy volunteers (Fig. 1F). Data represent means ±SE from 10 healthy individuals and 10 individuals with T2DM.