Canonical Wnt Pathway Inhibitor ICG-001 Induces Cytotoxicity of Multiple Myeloma Cells in Wnt-Independent Manner
Fig 2
Treatment with ICG-001 decreases viability and induces growth arrest of MM cells.
(A) MM cells were treated with vehicle control or indicated concentrations of ICG-001 for 24 hours. Cell viability was determined by MTT assay. (B, C) MM H929 cells were treated with indicated concentrations of ICG-001 for 24 or 48 hours. Cells were then collected, fixed, and stained with propidium iodide. Cell cycle distribution (B) and the proportion of cells in subG1 phase (C) were determined. Experiment was performed twice. Results of one experiment performed in triplicates are shown. *P<0.05, **p<0.01, and +p<0.005, as compared to vehicle control group. (D, E) Apoptosis of MM cell lines (D) or primary CD138+ MM cells (E) treated with ICG-001 for 24 hours (10 μM for RPMI-8226 and 40 μM for other cell lines) was evaluated using Annexin V binding assay by flow cytometry. In D, experiment was repeated 3 times with similar results, with results shown from one representative experiment performed in triplicate. *P<0.000001. In E, combined results for primary MM cells obtained from 4 MM patients are shown.