Aberrant DNA Damage Response Pathways May Predict the Outcome of Platinum Chemotherapy in Ovarian Cancer
Fig 5
The induction of the apoptotic pathway in carboplatin-treated PBMCs.
(A) Box plots showing statistical distribution of the individual apoptosis rates, expressed as doses of carboplatin inducing apoptosis in the three groups of individuals. HV, healthy volunteers; Sens: OC patients sensitive to subsequent platinum therapy; Res: OC patients resistant to subsequent platinum therapy. The correlations between the individual apoptosis rates and the DNA damage in PBMCs from the same individuals using γH2AX immunofluorescence staining (B) and comet assay (C) are presented. (D) Box plots showing statistical distribution of the levels of the pan-nuclear immunofluorescence γH2AX staining in the three groups of individuals. The horizontal lines within the boxes represent the median values and the vertical lines extending above and below the box indicate maximum and minimum values, respectively. (E) Correlation between pan-nuclear γH2AX staining and the individual apoptosis rates in the same samples. (F) Typical images showing pan-nuclear immunofluorescence γH2AX staining; upper images, γH2AX staining; bottom images, cell nuclei labeled with DAPI. All assays were performed in triplicate.