HuD Interacts with Bdnf mRNA and Is Essential for Activity-Induced BDNF Synthesis in Dendrites
Figure 3
PMA treatment leads to PKC-dependent increases in dendritic translation of local reporter constructs with the long Bdnf 3' UTR.
(A) Schematic representing the constructs used. (B and C) Colorized dendritic GFP fluorescence from dissociated rat hippocampal cultures transfected at 14 DIV with the indicated constructs and on 16 DIV treated for 1 hour with KCl, PMA or vehicle, fixed and then imaged (scale bar, 20 μm). (D and E) Mean ± SEM in 50-μm bins from images represented in (B) and (C) (n = 25; *, p<0.05; **, p<0.01; student's t-test). (F) Colorized dendritic GFP fluorescence from hippocampal cultures transfected at 14 DIV with ExIV A*B and on 16 DIV pre-treated for 30 min with GF109203X, a PKC inhibitor, then treated for 1 hour with KCl or PMA, fixed and then imaged (scale bar, 20 μm). (G) Mean ± SEM in 50-μm bins from images represented in (F) (n = 25; *, p<0.001; student's t-test).