Global 3D Imaging of Yersinia ruckeri Bacterin Uptake in Rainbow Trout Fry
Fig 7
Immunohistochemistry staining of Y. ruckeri bacterin in gill of vaccinated trout.
Gill sections from whole fish fixed in Carnoy’s fixative solution and used for immunostaining of Y. ruckeri bacterin. The sections are from (A) non-vaccinated, (B) 30 seconds post vaccination, (C) 5 minutes post vaccination, (D) 30 minutes post vaccination, (E) 3 hours post vaccination and (F) 24 hours post vaccination, and were all immunostained with anti-Y. ruckeri polyclonal antibody as described in materials and methods.