Investigating the Cellular Distribution and Interactions of HIV-1 Nucleocapsid Protein by Quantitative Fluorescence Microscopy
Fig 3
FRAP experiments in eGFP and NCp7-eGFP expressing cells.
(A) Time lapse sequence of typical FRAP measurements in the cytoplasm of an NCp7-eGFP expressing cell. The bleached region is highlighted by the red circle. Normalized fluorescence recovery curves of (B) eGFP and (C) NCp7-eGFP in the cytoplasm. For both eGFP and NCp7-eGFP, the distribution of residuals indicated a much better fit of the recovery curves with a double exponential as compared to a single exponential fit.