Tyrosine Hydroxylase Is Short-Term Regulated by the Ubiquitin-Proteasome System in PC12 Cells and Hypothalamic and Brainstem Neurons from Spontaneously Hypertensive Rats: Possible Implications in Hypertension
Fig 3
Colocalization of TH with ubiquitin or proteasome in PC12 cells.
Cells were incubated with either mouse anti-TH plus rabbit anti-ubiquitin antibodies (upper panel) or rabbit anti-TH plus mouse anti-proteasome antibodies (lower panel) followed by incubation with a anti-rabbit antibody coupled to Alexa 488 and anti-mouse coupled to AlexaFluor 594. See text for details. Scale bar = 5 μm. Three separate cell culture preparations were performed and 10 different images were taken and analyzed for each experiment. One representative image is presented.