The N-Terminal Residues 43 to 60 Form the Interface for Dopamine Mediated α-Synuclein Dimerisation
Fig 4
Stability of DA:α-syn 43–140 dimer exposed to denaturants.
Silver stain SDS-PAGE gel of DA:α-syn 43–140 dimer incubated with different amounts of denaturants HFIP or TFE. The dimers did not dissociate under any of the conditions tested, suggesting a covalent cross-link. Lane 1: DA:α-syn 43–140 dimer + 1% HFIP. Lane 2: DA:α-syn 43–140 dimer + 2% HFIP. Lane 3: DA:α-syn 43–140 dimer + 4% HFIP. Lane 4: DA:α-syn 43–140 dimer + 5% HFIP. Lane 5: DA:α-syn 43–140 dimer + 4% TFE. Lane 6: DA:α-syn 43–140 dimer + 8% TFE. Lane 7: DA:α-syn 43–140 dimer + 10% TFE. Lane 8: DA:α-syn 43–140 dimer + 15% TFE. Arrow indicates the DA:α-syn 43–140 dimer band.