A Membrane-Type-1 Matrix Metalloproteinase (MT1-MMP) – Discoidin Domain Receptor 1 Axis Regulates Collagen-Induced Apoptosis in Breast Cancer Cells
Fig 5
Transcriptomic alterations induced by MT1-MMP in MCF-7 cells embedded in 3D COL1.
Control (CTRL) and MT1-MMP (MT1) expressing MCF-7 cells were cultured for 24, 48 and 72h in 3D COL1. RNA was extracted from each sample and gene expression values measured using the Illumina Human HT-12 BeadChip array. (A) Probes with a fold change ≥ 1.8 between CTRL and MT1 cells in at least 1 point in the time course were selected and displayed as a heat map based on unsupervised hierarchical clustering. Red colour indicates genes that were up-regulated and green colour indicates genes that were down-regulated. Black indicates genes whose expression is unchanged in MT1 cells as compared to CTRL cells. (B) Venn diagram showing the overlap of modulated genes at the three time points. Numbers in italics, red, and underlined represent up-, contra-, and down-regulated genes, respectively. Numbers in brackets refer to the numbers of genes modulated at each time point. Venn analyses were performed using gene symbols. (C) Bio-function analysis of the dataset of genes differentially regulated upon MT1-MMP expression was performed in IPA. The significance is expressed as a negative log of p-value, which was calculated using the right-tailed Fisher's Exact Test. The orange line represents the threshold p value of 0.05.