Generation of a Persistently Infected MDBK Cell Line with Natural Bovine Spongiform Encephalopathy (BSE)
Figure 5
Comparison of the mobilities of PK-resistant PrP derived from BSE-infected bovine brain (original cell inoculate) and M2B cell.
Mobility phases of nonglycosylated PrPBSE from the non-infected bovine brain (lane 1), BSE-infected bovine brain (lane 2), non-infected transduced MDBK (MDBK C1–2F, passage (p) 7: lane 3), and persistently PK-resistant PrPBSE infected M2B cells (p17: lane 4; p48: lane 5 and p70: lane 6) in Western blotting after sequential treatments with PK and PNGase F. Molecular mass marker (M) in kilodaltons (kDa) is shown on the left.