GSK3β Is Increased in Adipose Tissue and Skeletal Muscle from Women with Gestational Diabetes Where It Regulates the Inflammatory Response
Figure 8
Effect of GSK3 inhibitor CHIR99021 on LPS-induced adhesion molecules in adipose tissue and skeletal muscle.
Human (A–D) omental adipose tissue and (E–H) skeletal muscle were incubated with 10 µg/ml LPS in the absence or presence of 10 µM CHIR99021 (CHIR) for 20 h (n = 6 patients). (A,B,E,F) Gene expression for VCAM-1 and ICAM-1 was analysed by qRT-PCR. Gene expression was normalised to GAPDH mRNA expression and the fold change was calculated relative to LPS. Data displayed as mean ±SEM. *P<0.05 vs. LPS (one-way ANOVA). (C,D,G,H) The incubation medium was assayed for concentration of sVCAM-1 and sICAM-1 release by ELISA. Data displayed as mean ±SEM. *P<0.05 vs. LPS (one-way ANOVA).