Ginsenoside Rg3 Inhibits Melanoma Cell Proliferation through Down-Regulation of Histone Deacetylase 3 (HDAC3) and Increase of p53 Acetylation
Figure 6
Down-regulation of HDAC3 by Rg3 alters cell cycle protein expression.
A375 cells were treated with HDAC3 siRNA, Rg3 (50 µg/mL), MS-275 (10 µM), or HDAC3 siRNA transfection followed by Rg3 treatment. Control: untransfected cells; Mock: cells transfected with vector. (A) Western blotting analysis of expression levels of PRB, cyclin E, cyclin D1, CDK4, CDK2 and p21. GAPDH was used as an internal control. (B) The statistical analyses for Western blotting results were shown. Data were presented as the mean±S.D. of three independent experiments. *, p<0.05; **, p<0.01.