Identification of c-MYC SUMOylation by Mass Spectrometry
Figure 7
MCF10A cells were treated with 4OHT for 24 hours to stimulate transgene expression. Cells were then either treated with vehicle (DMSO) or MG132 for 4 hours prior to harvest and immunoprecipitation for MYC. Immunoblotting was performed for SUMO1 and SUMO2/3 revealing that SUMOylation of MYC was enhanced following proteasomal inhibition. This effect was independent of the amount of MYC present in the pulldown (lower panel).