Early Life Stress Inhibits Expression of Ribosomal RNA in the Developing Hippocampus
Figure 2
rRNA levels in the hippocampus peak during the second week of life in a manner that is associated with reduced DNA methylation at the rDNA promoter.
A. rRNA levels peak during the second week of life. B. (top), a representative pyrogram of the pyrosequencing assay used to assess DNA methylation (blue) lines, (bottom) schematic diagram of the rDNA promoter elements. C. DNA methylation at the rDNA is lower in PND14 compared to PND7 and PND63 for CG_3 and CG_6. D. DNA methylation levels at CG_6 on PND7, 14, and 63. One-way ANOVA followed by Tukey-post hoc analysis when compared to PND14 (A, D), repeated measures ANOVA followed by simple-effect analysis (C). Error bars represent mean ± SEM, *p<0.05. UCE-upstream core element, To- Proximal-promoter terminator sequence.