HSPD1 Interacts with IRF3 to Facilitate Interferon-Beta Induction
Figure 3
Overexpression of HSPD1 facilitated IFN-β induction.
A. The expression of Myc-tagged HSPD1 was detected using an antibody against the Myc tag. B and E. The HEK293T cells were co-transfected with the luciferase reporter plasmid pIFN-β-Luc or pIRF3-Luc, the Renilla luciferase plasmid phRL-TK and plasmid encoding Myc-tagged HSPD1 or control vector. After incubation for 24 h, the cells were infected with SeV or mock-treated with the same buffer. After infection for 8 h, all of the cells were collected and the luciferase activity was measured using a dual-luciferase assay system. Data represent the relative firefly luciferase activity normalized to the Renilla luciferase activity. C and F. The HEK293T cells were co-transfected with 200 ng of the luciferase reporter plasmid pIFN-β-Luc or pIRF3-Luc, 20 ng of the Renilla luciferase plasmid phRL-TK (Promega), 200 ng of plasmid encoding Myc-tagged HSPD1 or control vector, and 200 ng of plasmid encoding RIG-IN or control vector for 36 h. The cells were then collected, and the luciferase activity was measured using a dual-luciferase assay system (Promega) and a luminometer (Turner BioSystems, CA). D. The HEK293T cells were co-transfected with 200 ng of the luciferase reporter plasmid p NF-κB -Luc or pIRF3-Luc, 20 ng of the Renilla luciferase plasmid phRL-TK (Promega), 400 ng of plasmid encoding Myc-tagged HSPD1 or control vector. After incubation for 24 h, the cells were infected with SeV or mock-treated with the same buffer. After infection for 8 h, all of the cells were collected and the luciferase activity was measured using a dual-luciferase assay system.