HSPD1 Interacts with IRF3 to Facilitate Interferon-Beta Induction
Figure 2
Co-localization of IRF3 and HSPD1.
A. HeLa cells were transfected with the MAVS or control plasmid. At 8 h post-transfection, the cells were fixed, permeabilized, and then stained with rabbit antibody against IRF3 and mouse antibody against HSPD1 and further incubated with goat anti-mouse IgG H&L (Cy3) and goat anti-rabbit IgG H&L (FITC). Nuclei were stained with DAPI. B. HeLa cells were transfected with the MAVS or control plasmid. At 16 h post-transfection, the cells were fixed, permeabilized, and then stained with rabbit antibody against IRF3 (phospho S386) and mouse antibody against HSPD1 and further developed with goat anti-mouse IgG H&L (Cy3) and goat anti-rabbit IgG H&L (FITC). Nuclei were stained with DAPI.