Expression and Evolution of the Non-Canonically Translated Yeast Mitochondrial Acetyl-CoA Carboxylase Hfa1p
Figure 1
Identification of the HFA1 transcription initiation site by 5′RACE: The HFA1 specific primer SP1 (Table 4) was used for the reverse transcription reaction to synthesize a first strand cDNA.
a) Major 5′ RACE product (500 bp), b) Minor 5′RACE product (650 bp) and c) third band produced only occasionally.