Implication of TLR- but Not of NOD2-Signaling Pathways in Dendritic Cell Activation by Group B Streptococcus Serotypes III and V
Figure 3
Impact of MyD88 on cytokine release by DCs in response to GBS.
Control (CTRL) or MyD88-/- bmDCs were stimulated with GBS wild-type (WT) strains or their respective non-encapsulated (ΔcpsE) mutants (106 CFU/ml, initial MOI:1). After 2 h of bmDC-GBS infection, a bacteriostatic agent (chloramphenicol, 12 µg/ml) was added to the culture to prevent cell toxicity. Non-stimulated cells served as negative control (C-) for basal expression levels. Supernatants were harvested at 16 h of incubation and cytokine production quantified by ELISA. Data are expressed as mean ± SEM (in ng/ml) from eight independent experiments. * P<0.05, indicate statistically significant differences between WT strains and their respective non-encapsulated mutants.