Nitric Oxide-Mediated Antioxidative Mechanism in Yeast through the Activation of the Transcription Factor Mac1
Figure 2
ChIP analysis of yeast cells treated with the exogenous NO.
S. cerivisiae BY4741 and BY4741MAC1-TAP cells grown in SD medium with/without NO treatment or in LCM medium, which is the same as SD medium without supplementation of copper, were subjected to ChIP analysis. (A) Electrophoresis of amplified DNA corresponding to the promoter region of each gene. The CMD1 gene, which is not under the control of Mac1, was used as a negative control. (B) Quantification of DNA binding to Mac1 by densitometry. The signal from immunoprecipitated DNA was divided by the signal from the corresponding input DNA. The resultant values were normalized as the value in SD medium is 100%.