Ets and GATA Transcription Factors Play a Critical Role in PMA-Mediated Repression of the ckβ Promoter via the Protein Kinase C Signaling Pathway
Figure 6
Effects of PMA concentration and treatment duration on ckβ promoter activity.
(A) MCF-7 cells were treated with 10, 20, or 30 ng/mL PMA for 6 hr. Each bar represents the mean ±SEM of triplicate samples from three independent experiments. (*p<0.05 vs. DMSO control; #p<0.05, within the PMA treatment group). (B) MCF-7 cells were treated with 20 ng/mL of PMA for 6 to 72 hr. The values shown are ckβ promoter activities relative to their respective controls (control value is set at 1.0, *p<0.05 vs. DMSO control). Each bar represents the mean ±SEM of triplicate samples from three independent experiments. (C) Effect of PMA treatment on the ckβ mRNA and protein levels in MCF-7 cells. Cells were treated with 20 ng/mL of PMA or DMSO for 6 to 24 hr. Left panel: The levels of mRNA and proteins are relative to their respective controls (control value is set at 1.0, *p<0.05 vs. DMSO control). Each bar represents the mean ±SEM of triplicate samples from three independent experiments. Right panel: The blot shown is representative of three independent experiments that produced similar results. M: SuperSignal Molecular Weight protein ladders (Thermo Scientific, USA), C: control, T: PMA treated, P: purified CKβ protein (45 kDa) as reference.