Evaluation of the Efficacy & Biochemical Mechanism of Cell Death Induction by Piper longum Extract Selectively in In-Vitro and In-Vivo Models of Human Cancer Cells
Figure 2
PLX Selectively Induces Cell Death in Human Cancer Cells in a Dose & Time Dependent Manner.
(A) Following treatment of Human pancreatic (BxPc-3) cancer and T cell leukemia cells with PLX, at indicated time points, cells were incubated with propidium iodide and assessed for the induction of cell death by image-based cytometry. (B) Similar experiments were carried out in human colon cancer cells (HT-29) and normal colon epithelial cells (NCM460). Fluorescence microscopy was used to assess the induction of cell death as characterized by presence of propidium iodide positive cells. Images were taken at 400× magnification on a fluorescent microscope. Scale bar = 15 µm.