Differential Roles of ERα and ERβ in Normal and Neoplastic Development in the Mouse Mammary Gland
Figure 2
Effects of pretreatment with EGF and progesterone on the mammary gland development in MMOC.
WT, αERKO and βERKO mice were treated with 25 ng EGF and 1 mg Progesterone for 5 days s.c. The glands were dissected and cultured in serum free Weymouth’s medium for 10 days in the presence of insulin (1 µg/ml), prolactin (5 µg/ml), aldosterone (1 µg/ml) and hydrocortisone (1 µg/ml). Results show that mice pretreated with EGF and P can replace pretreatment with Estradiol and progesterone for ERKO mice. A. WT, B. αERKO, C. βERKO Mice.