Efficacy of Combined Vancomycin and Fosfomycin against Methicillin-Resistant Staphylococcus aureus in Biofilms In Vivo
Figure 2
The experiment design employed in this study.
The rat sodium carboxymethylcellulose (CMC)-pouch MRSA biofilm infection model was established in twenty-four rats for each strain. On day 0, the CMC pouch was formed by injecting CMC and MRSA strains KZ306, ATCC43300 or Staphylococcus epidermidis strain ATCC35984. On Days 4 and 8, four rats were euthanized, and the pouches were excised for histology examination. On Day 9 after the bacterial infection, the remaining 16 rats for each strain were randomly divided into four groups to receive antibiotics therapy or saline for 7 days. On Day 16, all rats were euthanized and pouches, blood samples and exudates from the pouches were collected for examination.