Cell Surface Protein Disulfide Isomerase Regulates Natriuretic Peptide Generation of Cyclic Guanosine Monophosphate
Figure 3
PDI effects on cGMP generation of LLC-PK1 cells stimulated by ANP.
A. PDI expression in vascular smooth muscle cells (VSMC), endothelial cells (HUVEC), and mesangial cells (HMC). Expression of PDI was not detected on LLC-PK1 cells (porcine renal tubular cells). B. Bacitracin had no effect on cGMP generation of LLP-CK1 cells stimulated by ANP. C. Addition of purified PDI increased cGMP production significantly in LLC-PK1 cells compared to the cells without PDI. Data are expressed as mean+SEM vs control without PDI addition (*p value ≤0.05). (Error bars, +SD from 3 independent experiments, samples were triplicated in each experiment).