Otitis Media in Sperm-Associated Antigen 6 (Spag6)-Deficient Mice
Figure 6
Moraxella catarrhalis is detected both in the mutant and wild-type mice.
(A–B) PCR results and bacterial colony count results. (A) Specific primers for Streptococcus pneumonia (S.p), Haemophilus influenza (H.inf), and Moraxella catarrhalis (M.cata) were used to detect the bacteria in the middle ear. Bacteria obtained from ATCC were used as the reference. Moraxella catarrhalis was detected (237 bp, n = 4 for each group) in the tympanic washing fluid from both of the 25 day old mutant mice and control mice. Moraxella catarrhalis was also detected in cultures in both wild-type mice and mutant mice, which was consistent with PCR results. The colony count results showed that there was no significant difference in quantity of bacterium between the two groups (P>0.05). Streptococcus pneumonia (484 bp) and Haemophilus influenza (525 bp) were undetectable. +/+, the wild-type mice; −/−, the mutant mice; TF, tympanum fluid; C, culture.