Design and Validation of DNA Libraries for Multiplexing Proximity Ligation Assays
Figure 6
PLA template library screen with the solid phase proximity ligation assay (spPLA) system.
The matrix shows the Z-scores for the PLA reactions of 64 antibody pairs with the same adaptors as in Figure 4 screened against eight coding connectors (CC) from the in silico generated PLA templates. The detection connector sequence was kept constant in all rolling-circle amplification (RCA) reactions. Diagonal elements of the matrix (white bars) are the eight matched adaptor/connector pairs. The numbering of the adaptor and connector is the same as the numbering of the PLA templates from Figure 4B. The plates 4 and 8 were removed in this screening because of false-positive reaction results in the RCA screening. The lower histogram presents the absolute values of the Z-scores for the complete screening.