L-Histidine Inhibits Biofilm Formation and FLO11-Associated Phenotypes in Saccharomyces cerevisiae Flor Yeasts
Figure 3
Biofilm formation of flor strains is inhibited by L-histidine.
(A) Biofilm formation at the air-liquid interface in 24-well microtiter plates for strains A9, M23, V80, 3238-32, 3238-32Δflo11 and S288c after 5 days of static incubation in 1.5 mL flor medium at 30°C in the absence (Ctrl) and presence of 10 mM of L-histidine (L-his) and L-histidine–containing dipeptides. The biofilm is visualized as opaque floating material at the top of each well. (B) Dry weight determinations of the biofilms formed by the strains in (A) without (Ctrl) and with treatment with 10 mM L-histidine and the L-histidine–containing dipeptides (as indicated). Data are means +SD of three replicate treatments. (C) CFU recovery after plating on YPD agar using serial dilutions of a duplicate of all the strains/L-histidine and strains/dipeptides combinations.