Influence of Trichobilharzia regenti (Digenea: Schistosomatidae) on the Defence Activity of Radix lagotis (Lymnaeidae) Haemocytes
Figure 8
Effect of PKC (GF109203X) and MEK (U0126) inhibitors on phagocytosis by haemocytes from uninfected Radix lagotis.
Haemocyte monolayers were pre-incubated with (A) GF109203X, (B) U0126, or vehicle (DMSO; shown as controls) prior to challenge with E. coli bioparticles. The intracellular fluorescence resulting from phagocytosis was measured using a microplate reader and mean (± SEM; n = 7) levels of phagocytosis expressed in relation to control (100%) values. **p<0.01, ***p<0.001, when compared to control values; paired t-test.