A Critical Role of CDKN3 in Bcr-Abl-Mediated Tumorigenesis
Figure 2
Overexpression of CDKN3 dramatically inhibits K562 xenografted tumor growth in nude mice.
(A) Nude mice were subcutaneously injected with K562 cells stably expressing CDKN3-WT or EV. The tumor volumes were measured at indicated time points. Plotted are results from three independent experiments. Error bars, SEM; n = 9; *P<0.05. (B) Tumors were excised from mice. Shown are representative images from four independent experiments with similar results. (C) Relative volume of tumors excised from nude mice injected with K562 cells expressing CDKN3-WT or EV (control). The average volume of control tumors is set to 100%. Error bars, SEM; n = 9; *P<0.05. (D) Over a 21-day period after inoculation, tumors formed by control or CDKN3-WT overexpressing K562 cells were measured by bioluminescent imaging. Shown are representative images from at least three independent experiments with similar results. (E) CDKN3 expression in representative tumors expressing CDKN3-WT or EV was examined by Western blotting. (F) Nude mice were subcutaneously injected with K562 cells stably expressing CDKN3-C140S or EV. The tumor volumes were measured at indicated time points. Shown are volumes of tumors excised from nude mice injected with K562 cells expressing CDKN3-C140S or EV. Plotted are results from three independent experiments. Error bars, SEM; n = 9. (G) Tumors from nude mice injected with K562 cells expressing CDKN3-C140S or EV were excised from mice. Shown are representative images from three independent experiments with similar results.