Soluble LRIG2 Ectodomain Is Released from Glioblastoma Cells and Promotes the Proliferation and Inhibits the Apoptosis of Glioblastoma Cells In Vitro and In Vivo in a Similar Manner to the Full-Length LRIG2
Figure 7
LRIG2 as well as LRIG2ecto interacts with EGFR in glioblastoma cells.
(A) Confocal immunofluorescence laser microsopy showed colocalization of LRIG2 and EGFR in LRIG2 overexpresing U87 and U251 glioblastoma cells. Cells were stained with anti-LRIG2 and anti-EGFR antibodies, followed by using Cy3- and FITC-conjugated secondary antibodies. Yellow of merge panel indicates regions of colocalization. Scale bar, 50 µm. (B) Lysates from cells overexprssing LRIG2 or LRIG2ecto were immunoprecipitated with purified mouse anti-Flag monoclonal antibodies or control mouse IgG, and immunoprecipitates were blotted with antibodies to EGFR or Flag.