Soluble LRIG2 Ectodomain Is Released from Glioblastoma Cells and Promotes the Proliferation and Inhibits the Apoptosis of Glioblastoma Cells In Vitro and In Vivo in a Similar Manner to the Full-Length LRIG2
Figure 2
Establishment of glioblastoma cell lines stably expressing LRIG2 or LRIG2ecto.
(A) Schematic drawing of the domain organization of the Flag tagged full-length LRIG2 and LRIG2 ectodomain. Indicated are the signal peptides (SP), Flag tag (Flag), the leucine-rich repeat domains, consisting of cysteine-rich N-flanking domain (NF), 15 leucine-rich repeats (LRR) and cysteine-rich C-flanking domain (CF), three immunoglobulin-like domains (Ig-C2), the transmembrane domain (TM) and the cytoplasmic tail (Cyto). (B) The total cell lysates of stably transduced cells, cultured in complete medium for 48 h, were subjected to immunoblotting using an anti-Flag antibody. β-Actin served as an internal loading control. (C) After maintained in complete medium for 48 h, the cells were subjected to total RNA extraction, followed by quantitative RT-PCR to measure the LRIG2 and LRIG2ecto mRNA expression levels. Expressions are shown as the fold changes of control cells (**P<0.01, vs con). (D) After cultured in complete medium for 48 h, stably transduced cells were subjected to immunofluorescence analysis. Flag staining is green, and nuclear stain is blue. Representative images of three independent experiments were shown. Scale bars, 50 µm.